Muscone suppresses myocardial ischemia damage by regulating PI3K/Akt signaling pathway

Biochim Biophys Acta Mol Basis Dis. 2022 Dec 1;1868(12):166539. doi: 10.1016/j.bbadis.2022.166539. Epub 2022 Sep 11.

Abstract

Muscone is the main active compound of Moschus. In this paper, the cardioprotective effect of Muscone on acute myocardial ischemia (AMI) rats and its potential mechanisms were investigated. AMI rat models were established to evaluate the protective effect and antioxidative function of Muscone on the hearts. Moreover, Western blot analysis was conducted to quantify the phosphorylated PI3K and AKT levels in PI3K/Akt pathway for further investigating the mechanism of Muscone. Results showed that Muscone could markedly lessen the infarct size and myocardial injury, improve cardiac function, inhibit cardiomyocyte apoptosis and down-regulate serum reactive oxygen species level as indicated by the decreased MDA, BNP and c-TnI activities and the increased SOD, GSH-px, CAT activities and the expression of Bax protein. In addition, it was revealed that Muscone notably promoted the phosphorylation of PI3K and AKT. These findings denote that Muscone exerts a protective effect in heart via inhibition of oxidative stress and apoptosis, offering new insights into the treatment of CHD and the clinical application of Muscone.

Keywords: Apoptosis; Coronary heart disease; Muscone; Oxidative stress; PI3K/Akt pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cycloparaffins* / pharmacology
  • Myocardial Ischemia* / drug therapy
  • Phosphatidylinositol 3-Kinases / metabolism
  • Proto-Oncogene Proteins c-akt / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Reactive Oxygen Species / metabolism
  • Signal Transduction*
  • Superoxide Dismutase / metabolism
  • bcl-2-Associated X Protein / metabolism
  • bcl-2-Associated X Protein / pharmacology

Substances

  • Cycloparaffins
  • Reactive Oxygen Species
  • bcl-2-Associated X Protein
  • Superoxide Dismutase
  • Proto-Oncogene Proteins c-akt
  • muscone