Sattleria karsholti Huemer Hebert, 2011 is re-examined based on recently collected material, museum vouchers, and DNA barcodes. Adult and male genitalia morphology and molecular data of the COI barcode region support the existence of two allopatric species, S. karsholti, from the type locality of Pizo Arera in the Orobian Alps (Bergamo Province, Italy), and S. enrosadira sp. nov. from Monte Baldo, Adamello and the Brenta group (Trento and Verona Provinces, Italy). Adults and male genitalia of both species are figured; the females of both species are unknown.