Paracandidimonas lactea sp. nov., a urea-utilizing bacterium isolated from landfill

A urea-utilizing bacterium, designated Q2-2 ^T, was isolated from landfill. Cells of strain Q2-2 ^T were Gram stain-negative, aerobic, short-rod bacteria. Strain Q2-2 ^T was observed to grow at a temperature range of 15-37℃ (optimum 30 ℃), a pH range of 5.5-9.5 (optimum pH 8.0) and 0-4% (w/v) NaCl (optimum 1%). The major respiratory quinone was Q-8, and the major polar lipids were diphosphatidyl glycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, and phosphatidyl glycerol. Based on the 16S rRNA gene sequence, strain Q2-2 ^T had the highest similarity with Paracandidimonas caeni 24 ^T (98.0%), followed by Pusillimonas soli MJ07^T (97.5%), Parapusillimonas granuli Ch07^T (97.2%), Pusillimonas ginsengisoli DCY25^T (97.1%) and Paracandidimonas soli IMT-305 ^T (96.4%). The ANI values between strain Q2-2 ^T and the above related type strains were 71.02%, 73.52%, 74.32%, 74.59% and 72.29%, respectively. The DNA G + C content of strain Q2-2 ^T was 61.1%. Therefore, strain Q2-2 ^T represents a novel species of the genus Paracandidimonas, for which the name Paracandidimonas lactea sp. nov. (type strain Q2-2 ^T = CGMCC 1.19179 ^T = JCM 34906 ^T) is proposed.