Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

Maria Grazia Cascio; Pietro Marini; Roger G Pertwee

doi:10.1007/978-1-0716-2728-0_8

Displacement Binding Assay Using Human Cannabinoid CB₂ Receptor-Transfected Cells

Methods Mol Biol. 2023:2576:111-118. doi: 10.1007/978-1-0716-2728-0_8.

Authors

Maria Grazia Cascio¹, Pietro Marini¹, Roger G Pertwee²

Affiliations

¹ School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
² School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK. [email protected].

PMID: 36152180
DOI: 10.1007/978-1-0716-2728-0_8

Abstract

Displacement binding assays are nonfunctional assays mostly used with the aim of determining whether a certain compound (plant-derived or synthetic) can bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB₂ receptor.

Keywords: Affinity; Agonist; Antagonist; Binding; Cannabinoid CB2 receptors; Ligand; Radioligand.

MeSH terms

Animals
Biological Assay*
CHO Cells
Cannabinoids* / metabolism
Cricetinae
Cricetulus
Humans
Radioligand Assay
Receptor, Cannabinoid, CB2 / genetics
Receptors, Cannabinoid

Substances

Cannabinoids
Receptor, Cannabinoid, CB2
Receptors, Cannabinoid