Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

Pietro Marini; Maria Grazia Cascio; Roger G Pertwee

doi:10.1007/978-1-0716-2728-0_13

Cyclic AMP Assay Using Human Cannabinoid CB₂ Receptor-Transfected Cells

Methods Mol Biol. 2023:2576:171-179. doi: 10.1007/978-1-0716-2728-0_13.

Authors

Pietro Marini¹, Maria Grazia Cascio¹, Roger G Pertwee²

Affiliations

¹ School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
² School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK. [email protected].

PMID: 36152185
DOI: 10.1007/978-1-0716-2728-0_13

Abstract

The cyclic AMP assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, and inverse agonists) of G-protein coupled receptor ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available nonradioligand ready-to-use kits and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB₂ receptor.

Keywords: 3-Isobutyl-1-methylxanthine (IBMX); Agonist; Antagonist; Cannabinoid receptor type 2; Cyclic AMP; Forskolin (FSK); Inverse agonist.

MeSH terms

Animals
CHO Cells
Cannabinoids*
Cricetinae
Cricetulus
Cyclic AMP*
Humans
Ligands
Receptor, Cannabinoid, CB2 / genetics
Receptors, Cannabinoid

Substances

Cannabinoids
Ligands
Receptor, Cannabinoid, CB2
Receptors, Cannabinoid
Cyclic AMP