In some specific vascular plant tissues, lignin can impregnate the entire cell wall to make it more rigid and hydrophobic. Different techniques have been developed in the past years to make possible the quantification of this polyphenolic polymer at the organ or tissue level, but difficulties of access to the cellular level remain. Here we describe an approach based on ratiometric emission measurements using safranin-O and the development of a macro adapted for the FIJI software, which makes it possible to quantify lignin in three different layers of the cell wall on images captured on a fluorescent confocal microscope.
Keywords: Cell wall; Confocal microscopy; Lignin quantification; Safranin-O; Segmentation.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.