Epigenetic master regulators HDAC1 and HDAC5 control pathobiont Enterobacteria colonization in ileal mucosa of Crohn's disease patients

Mélissa Chervy; Adeline Sivignon; Flavie Dambrine; Anthony Buisson; Pierre Sauvanet; Catherine Godfraind; Matthieu Allez; Lionel Le Bourhis; The Remind Group; Nicolas Barnich; Jérémy Denizot

doi:10.1080/19490976.2022.2127444

Epigenetic master regulators HDAC1 and HDAC5 control pathobiont Enterobacteria colonization in ileal mucosa of Crohn's disease patients

Gut Microbes. 2022 Jan-Dec;14(1):2127444. doi: 10.1080/19490976.2022.2127444.

Authors

Mélissa Chervy¹, Adeline Sivignon^{1

2}, Flavie Dambrine¹, Anthony Buisson^{1

3}, Pierre Sauvanet^{1

4}, Catherine Godfraind^{1

5}, Matthieu Allez^{6

7

8}, Lionel Le Bourhis⁷, The Remind Group⁸, Nicolas Barnich^{1

2}, Jérémy Denizot^{1

2}

Affiliations

¹ Université Clermont Auvergne, Inserm U1071, USC-INRAE 2018, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Clermont-Ferrand, France.
² Institut Universitaire de Technologie, Génie Biologique, Aubière, France.
³ Gastroenterology Department, CHU Estaing, Clermont-Ferrand, France.
⁴ Surgery and Oncology Digestive Department, CHU Estaing, Clermont-Ferrand, France.
⁵ Neuropathology Unit, CHU Gabriel Montpied, Clermont-Ferrand, France.
⁶ Gastroenterology Department, Hôpital Saint-Louis - APHP, Paris, France.
⁷ Université De Paris, Institut de Recherche Saint-Louis, EMily, INSERM U1160, Paris, France.
⁸ Hôpital Saint-Louis, Paris, France.

Abstract

AIEC Adherent-Invasive Escherichia coli; BSA Bovine serum albumin; CD Crohn's disease; CEABAC10 Carcinoembryonic antigen bacterial artificial chromosome 10; CEACAM Carcinoembryonic antigen-related cell adhesion molecule; FBS Fetal bovine serum; IBD Inflammatory Bowel Disease; HAT Histone acetyltransferase; HDAC Histone deacetylase; kDa KiloDalton; SAHA Suberoylanilide Hydroxamic Acid; Scr Scramble.

Keywords: Adherent-InvasiveE. coli; Crohn’s disease; High-fat diet; Histone deacetylases; histone acetylation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoembryonic Antigen
Crohn Disease*
Enterobacteriaceae
Epigenesis, Genetic
Gastrointestinal Microbiome*
Histone Acetyltransferases / genetics
Histone Deacetylase 1
Histone Deacetylases
Humans
Mucous Membrane
Serum Albumin, Bovine
Vorinostat

Substances

Carcinoembryonic Antigen
Serum Albumin, Bovine
Vorinostat
Histone Acetyltransferases
HDAC1 protein, human
HDAC5 protein, human
Histone Deacetylase 1
Histone Deacetylases

Grants and funding

This study was supported by the Ministère de l’Enseignement supérieur, de la Recherche et de l’Innovation, Inserm (UMR 1071), INRAe (USC-2018), by the Fondation pour la Recherche Médicale (FRM, “Contrat doctoral,” ECO201906008953), by Agence Nationale de la Recherche. (ANR, Project ANR-21-CE14-0014-01), by the Association François Aupetit (AFA) and INRAE MICA department « Projet exploratoire » Institut National de la Santé et de la Recherche Médicale ”Programme Transversal Microbiote. The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.