Generation of CD16A gene knockout human embryonic stem cell line using CRISPR/Cas9

Shunjie Wu; Haimei Deng; Bo Lu; Lihua Yuan; Xiaobo Wang

doi:10.1016/j.scr.2022.102935

Generation of CD16A gene knockout human embryonic stem cell line using CRISPR/Cas9

Stem Cell Res. 2022 Oct:64:102935. doi: 10.1016/j.scr.2022.102935. Epub 2022 Oct 5.

Authors

Shunjie Wu¹, Haimei Deng², Bo Lu², Lihua Yuan³, Xiaobo Wang⁴

Affiliations

¹ Department of General Practice, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen, China.
² Department of Hematology, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen, China.
³ Department of Pediatric Surgery, University of Hong Kong-Shenzhen Hospital, Shenzhen, China. Electronic address: [email protected].
⁴ Department of Hematology, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen, China. Electronic address: [email protected].

PMID: 36219983
DOI: 10.1016/j.scr.2022.102935

Abstract

CD16A is a receptor for the Fc portion of immunoglobulin G, and is involved in the antibody dependent cellular cytotoxicity (ADCC) of nature killer cells(Zhu et al., 2020) and antibody dependent enhancement (ADE) of virus infections(Wan et al., 2020). However, the role of CD16A in human embryonic stem cell modeled development has been merely documented. Hence, to illustrate the role of CD16A in the human cell development, we reported a CD16A knockout human embryonic stem(hESC) cell line via CRISPR/Cas9 mediated gene knockout. The CD16A mutated cell line displayed normal karyotype, pluripotent stem cell marker gene expression and differentiation potential.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Cell Line
Gene Knockout Techniques
Human Embryonic Stem Cells* / metabolism
Humans
Immunoglobulin G / genetics
Immunoglobulin G / metabolism
Receptors, IgG / genetics

Substances

Receptors, IgG
Immunoglobulin G