99m Technetium-pyrophosphate bone scan: A potential biomarker for the burden of transthyretin amyloidosis in skeletal muscle: A preliminary study

Muscle Nerve. 2023 Feb;67(2):111-116. doi: 10.1002/mus.27740. Epub 2022 Oct 31.

Abstract

Introduction/aims: Transthyretin amyloidosis (ATTR) proteins can infiltrate skeletal muscle and infrequently cause a myopathy. 99m Technetium-pyrophosphate (99m Tc-PYP) is a validated biomarker for cardiac involvement in variant and wild-type ATTR (ATTRv and ATTRwt, respectively). The aim of this study was to test the hypothesis that 99m Tc-PYP is a biomarker for muscle burden of ATTR.

Methods: Radioisotope uptake in the deltoid muscles of patients with ATTR was compared to uptake in control subjects without amyloidosis in a retrospective study. 99m Tc-PYP scans were evaluated in 11 patients with ATTR (7 ATTRv, 4 ATTRwt) and 14 control subjects. Mean count (MC) values were measured in circular regions of interest (ROIs) 2.5-3.8 cm2 in area. Tracer uptake was quantified in the heart, contralateral chest (CC), and deltoid muscles.

Results: Tracer uptake was significantly higher over the deltoids and heart but not the CC, in patients with ATTR than in control subjects. MC values were 120.1 ± 43.7 (mean ± SD) in ATTR patients and 78.9 ± 20.4 in control subjects over the heart (p = 0.005), 73.3± 21.0 and 63.5 ± 14.4 over CC (p = 0.09), and 37.0 ± 11.7 and 26.0 ± 7.1 averaged over both deltoid muscles (p = 0.014).

Discussion: 99m Tc-PYP is a potential biomarker for ATTR amyloid burden in skeletal muscle.

Keywords: 99mTc-PYP; ATTR; amyloidosis; myopathy; transthyretin.

MeSH terms

  • Amyloid Neuropathies, Familial* / diagnostic imaging
  • Biomarkers
  • Cardiomyopathies*
  • Diphosphates
  • Humans
  • Muscle, Skeletal / diagnostic imaging
  • Prealbumin
  • Retrospective Studies
  • Technetium
  • Technetium Tc 99m Pyrophosphate

Substances

  • Technetium
  • diphosphoric acid
  • Diphosphates
  • Technetium Tc 99m Pyrophosphate
  • Biomarkers
  • Prealbumin

Supplementary concepts

  • Amyloidosis, Hereditary, Transthyretin-Related