"Genetic scissors" CRISPR/Cas9 genome editing cutting-edge biocarrier technology for bone and cartilage repair

Chao Li; Yawei Du; Tongtong Zhang; Haoran Wang; Zhiyong Hou; Yingze Zhang; Wenguo Cui; Wei Chen

doi:10.1016/j.bioactmat.2022.09.026

"Genetic scissors" CRISPR/Cas9 genome editing cutting-edge biocarrier technology for bone and cartilage repair

Bioact Mater. 2022 Oct 7:22:254-273. doi: 10.1016/j.bioactmat.2022.09.026. eCollection 2023 Apr.

Authors

Chao Li^{1

2}, Yawei Du², Tongtong Zhang¹, Haoran Wang^{1

2}, Zhiyong Hou¹, Yingze Zhang¹, Wenguo Cui², Wei Chen¹

Affiliations

¹ Department of Orthopaedics, The Third Hospital of Hebei Medical University, Orthopaedic Research Institution of Hebei Province, NHC Key Laboratory of Intelligent Orthopaedic Equipment, No.139 Ziqiang Road, Shijiazhuang, 050051, PR China.
² Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, PR China.

Abstract

CRISPR/Cas9 is a revolutionary genome editing technology with the tremendous advantages such as precisely targeting/shearing ability, low cost and convenient operation, becoming an efficient and indispensable tool in biological research. As a disruptive technique, CRISPR/Cas9 genome editing has a great potential to realize a future breakthrough in the clinical bone and cartilage repairing as well. This review highlights the research status of CRISPR/Cas9 system in bone and cartilage repair, illustrates its mechanism for promoting osteogenesis and chondrogenesis, and explores the development tendency of CRISPR/Cas9 in bone and cartilage repair to overcome the current limitations.

Keywords: Bone repair; CRISPR/Cas9; Cartilage repair; Genome editing.

Publication types

Review