A chimeric PCV rescued virus with the immunogenic cap gene of PCV3 cloned into the genomic backbone of the nonpathogenic PCV1 induces specific antibodies but with no pathogenic in pigs

Jianli Shi; Xiaoyan Wu; Shuo Wang; Jiaxin Li; Zhao Wang; Ying Yang; Zhe Peng; Chang Liu; Shaojian Xu; Chen Li; Yao Tian; Hong Han; Nataliia; Jun Li; Yongming Wang; Xianjie Han

doi:10.1016/j.micpath.2022.105839

A chimeric PCV rescued virus with the immunogenic cap gene of PCV3 cloned into the genomic backbone of the nonpathogenic PCV1 induces specific antibodies but with no pathogenic in pigs

Microb Pathog. 2022 Dec;173(Pt A):105839. doi: 10.1016/j.micpath.2022.105839. Epub 2022 Oct 18.

Authors

Jianli Shi¹, Xiaoyan Wu², Shuo Wang³, Jiaxin Li⁴, Zhao Wang⁵, Ying Yang⁶, Zhe Peng¹, Chang Liu¹, Shaojian Xu¹, Chen Li¹, Yao Tian⁴, Hong Han¹, Nataliia¹, Jun Li⁷, Yongming Wang⁸, Xianjie Han⁹

Affiliations

¹ Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, PR China.
² Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, PR China; College of Life Sciences, Shandong Normal University, Jinan, China.
³ College of Life Sciences, Shandong University, Qingdao, China.
⁴ Qingdao Agricultural University, Qingdao, China.
⁵ School of Laboratory Animal and Shandong Laboratory Animal Center, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.
⁶ College of Life Sciences, Shandong Normal University, Jinan, China.
⁷ Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, PR China; College of Life Sciences, Shandong Normal University, Jinan, China; Qingdao Agricultural University, Qingdao, China. Electronic address: [email protected].
⁸ Shandong Huahong Biological Engineering CO. LTD, Binzhou, China. Electronic address: [email protected].
⁹ Qingdao Agricultural University, Qingdao, China. Electronic address: [email protected].

PMID: 36265738
DOI: 10.1016/j.micpath.2022.105839

Abstract

A chimeric PCV called PCV1-3 with the immunogenic Cap gene of pathogenic PCV type 3(PCV3) cloned into the genomic skeleton of the nonpathogenic PCV1 was rescued and inoculated into PCV3 negative piglets. The results of fluorescence quantitative PCR showed that the PCV1-3 DNA detected in serum and tissues was negative. The pathogenicity of piglets showed that PCV 1-3 did not cause the clinical characteristics and pathological changes. The viral neutralization assay revealed that infected pigs could produce antibodies and neutralize the viral activity. All results showed that chimeric virus induced specific antibodies but with no pathogenic in pigs, which provided new candidate strains for the development of PCV3 vaccine.

Keywords: Chimeric virus; Porcine circovirus virus type 3; Rescue; Vaccine candidate; Viral neutralization.

MeSH terms

Animals
Antibodies, Viral
Circoviridae Infections* / prevention & control
Circoviridae Infections* / veterinary
Circovirus*
Genomics
Swine
Swine Diseases*
Viral Vaccines* / genetics

Substances

Viral Vaccines
Antibodies, Viral