Momordica charantia polysaccharide ameliorates D-galactose-induced aging through the Nrf2/β-Catenin signaling pathway

Metab Brain Dis. 2023 Mar;38(3):1067-1077. doi: 10.1007/s11011-022-01103-4. Epub 2022 Oct 26.

Abstract

Aging is widely thought to be associated with oxidative stress. Momordica charantia (MC) is a classic vegetable and traditional herbal medicine widely consumed in Asia, and M. charantia polysaccharide (MCP) is the main bioactive ingredient of MC. We previously reported an antioxidative and neuroprotective effect of MCP in models of cerebral ischemia/reperfusion and hemorrhage injury. However, the role played by MCP in neurodegenerative diseases, especially during aging, remains unknown. In this study, we investigated the protective effect of MCP against oxidative stress and brain damage in a D-galactose-induced aging model (DGAM). The Morris water maze test was performed to evaluate the spatial memory function of model rats. The levels of malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) were measured and telomerase activity was determined. The results showed that MCP treatment attenuated spatial memory dysfunction induced by D-galactose. In addition, MCP increased antioxidant capacity by decreasing MDA and increasing SOD and GSH levels. MCP treatment also improved telomerase activity in aging rats. Mechanistically, MCP promoted the entry of both Nrf2 and β-Catenin into the nucleus, which is the hallmark of antioxidation signaling pathway activation. This study highlights a role played by MCP in ameliorating aging-induced oxidative stress injury and reversing the decline in learning and memory capacity. Our work provides evidence that MCP administration might be a potential antiaging strategy.

Keywords: Aging; Momordica charantia polysaccharide; Nrf2; Oxidative stress; β-Catenin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / metabolism
  • Animals
  • Antioxidants / metabolism
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use
  • Galactose / toxicity
  • Malondialdehyde / metabolism
  • Momordica charantia* / metabolism
  • NF-E2-Related Factor 2 / metabolism
  • Oxidative Stress
  • Polysaccharides / pharmacology
  • Polysaccharides / therapeutic use
  • Rats
  • Signal Transduction
  • Superoxide Dismutase / metabolism
  • Telomerase* / metabolism
  • Telomerase* / pharmacology
  • beta Catenin / metabolism

Substances

  • Galactose
  • NF-E2-Related Factor 2
  • beta Catenin
  • Telomerase
  • Antioxidants
  • Polysaccharides
  • Superoxide Dismutase
  • Malondialdehyde