Characteristics of carbon, nitrogen, phosphorus and sulfur cycling genes, microbial community metabolism and key influencing factors during composting process supplemented with biochar and biogas residue

Nanyi Wang; Keqi Zhao; Fanghong Li; Hua Peng; Yaoxiong Lu; Lihua Zhang; Junting Pan; Shilin Jiang; Anwei Chen; Binghua Yan; Lin Luo; Hongli Huang; Hui Li; Genyi Wu; Jiachao Zhang

doi:10.1016/j.biortech.2022.128224

Characteristics of carbon, nitrogen, phosphorus and sulfur cycling genes, microbial community metabolism and key influencing factors during composting process supplemented with biochar and biogas residue

Bioresour Technol. 2022 Dec:366:128224. doi: 10.1016/j.biortech.2022.128224. Epub 2022 Oct 31.

Authors

Nanyi Wang¹, Keqi Zhao¹, Fanghong Li², Hua Peng³, Yaoxiong Lu³, Lihua Zhang¹, Junting Pan⁴, Shilin Jiang⁵, Anwei Chen¹, Binghua Yan¹, Lin Luo¹, Hongli Huang¹, Hui Li⁴, Genyi Wu², Jiachao Zhang⁶

Affiliations

¹ College of Resources and Environment, Hunan Agricultural University, Changsha 410128, Hunan, China.
² College of Resources and Environment, Hunan Agricultural University, Changsha 410128, Hunan, China; State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment of the PR China, Guangzhou 510655, China.
³ Institute of Agricultural Environment and Ecology, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, China.
⁴ Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
⁵ College of Resources and Environment, Hunan Agricultural University, Changsha 410128, Hunan, China; State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410029, China.
⁶ College of Resources and Environment, Hunan Agricultural University, Changsha 410128, Hunan, China. Electronic address: [email protected].

PMID: 36328174
DOI: 10.1016/j.biortech.2022.128224

Abstract

Carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) cycling functional genes and bacterial and fungal communities during composting with biochar and biogas residue amendments were studied. Correlations between microbial community structure, functional genes and physicochemical properties were investigated by network analysis and redundancy analysis. It was shown that the gene of acsA abundance accounted for about 50% of the C-related genes. Biogas residue significantly decreased the abundance of denitrification gene nirK. Biogas residues can better promote the diversity of bacteria and fungi during composting. Biochar significantly increased the abundance of Humicola. Redundancy analysis indicated that pile temperature, pH, EC were the main physicochemical factors affecting the microbial community. WSC and NO₃^--N have significant correlation with C, N, P, S functional genes. The research provides a theoretical basis for clarifying the metabolic characteristics of microbial communities during composting and for the application of biochar and biogas residues in composting.

Keywords: Biochar; Biogas residue; Composting; Functional gene; Microbial community metabolism.

MeSH terms

Bacteria / genetics
Bacteria / metabolism
Biofuels
Carbon
Charcoal
Composting*
Microbiota* / genetics
Nitrogen / metabolism
Phosphorus
Soil / chemistry
Sulfur

Substances

biochar
Nitrogen
Biofuels
Phosphorus
Carbon
Soil
Charcoal
Sulfur