Detection of abemaciclib, an anti-breast cancer agent, using a new electrochemical DNA biosensor

Zimeng Lei; Merim Alwan; Hassan Thoulfikar A Alamir; Hussein Humedy Chlib Alkaaby; Sinan Subhi Farhan; Sura A Awadh; Usama S Altimari; Hawra'a Fadhel Abbas Al-Baghdady; Athmar Ali Kadhim; Maytham T Qasim; Ali Hussein Adhab; Abuzar Nekuei

doi:10.3389/fchem.2022.980162

Detection of abemaciclib, an anti-breast cancer agent, using a new electrochemical DNA biosensor

Front Chem. 2022 Oct 21:10:980162. doi: 10.3389/fchem.2022.980162. eCollection 2022.

Affiliations

¹ School of International Education, Beijing University of Chemical Technology, Beijing, China.
² Medical Lab. Techniques Department, College of Medical Technology, Al-Farahidi University, Baghdad, Iraq.
³ Faculty of Pharmacy Department of Pharmaceutics, University of Al-Ameed, Karbala, Iraq.
⁴ Al-Manara College for Medical Sciences, Maysan, Iraq.
⁵ The University of Mashreq, Baghdad, Iraq.
⁶ Department of Anesthesia, Al-mustaqbal University, Babylon, Iraq.
⁷ Al-Nisour University College, Baghdad, Iraq.
⁸ Hawra'a Fadhel Abbas AL-Baghdady, College of Dentistry, The Islamic University, Najaf, Iraq.
⁹ Medical Laboratories Teachniques, Hilla University College Babylon, Babylon, Iraq.
¹⁰ Department of Anesthesia, College of Health and Medical Technology, Al-Ayen University, Thi-Qar, Iraq.
¹¹ Department of Medical Laboratory Technics, Al-Zahrawi University College, Karbala, Iraq.
¹² Islamic Azad University of South Tehran Branch, Tehran, Iran.

Abstract

Detection of DNA molecules and possible chemotherapy-induced changes in its structure has been the goal of researchers using rapid, sensitive and inexpensive approaches. Therefore, the aim of this study was to fabricate a new electrochemical DNA biosensor using pencil graphite electrodes modified with polypyrrole/Ce doped hexagonal nickel oxide nanodisks or PP/Ce-doped H-NiO-ND composites for determination of Abemaciclib (AMC) and ds-DNA molecules. The DNA biosensor was prepared by immobilizing ds-DNA on the surface of PP/Ce-doped H-NiO-ND/PGE. Differential pulse voltammetry (DPV) was used to electrochemically detect AMC. The results elucidate the extremely high sensitivity of the ds-DNA/PP/Ce-doped H-NiO-ND/PGE biosensor to AMC, with a narrow detection limit of 2.7 nM and a lengthy linear range of 0.01-600.0 μM. The admirable performance of as-fabricated biosensor could be related to the active reaction sites and the unique electrochemical response related to the nanocomposites by enhancing ds-DNA stabilization and accelerating electron transfer on the surface of electrode.

Keywords: DNA biosensor; abemaciclib; anti-breast cancer agent; modified electrode; voltammetry.