Receptor-ligand non-equilibrium kinetics (RLNEK) 1.0: An integrated Trackmate laminar flow chamber analysis

Zachary A Rollins; Allison Chan; Venktesh S Shirure; Steven C George

doi:10.1016/j.jim.2022.113381

Receptor-ligand non-equilibrium kinetics (RLNEK) 1.0: An integrated Trackmate laminar flow chamber analysis

J Immunol Methods. 2022 Dec:511:113381. doi: 10.1016/j.jim.2022.113381. Epub 2022 Oct 27.

Authors

Zachary A Rollins¹, Allison Chan¹, Venktesh S Shirure², Steven C George³

Affiliations

¹ Department of Chemical Engineering, University of California, Davis, Davis, CA, USA.
² Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA.
³ Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA. Electronic address: [email protected].

PMID: 36341963
DOI: 10.1016/j.jim.2022.113381

Abstract

Although parallel plate flow chamber assays are widely performed, extraction of kinetic parameters is limited to specialized labs with mathematical expertise and customized video-microscopy tracking tools. The recent development of Trackmate has increased researcher accessibility to tracking particles in video-microscopy experiments; however, there is a lack of tools that analyze this tracking information. We report a software tool, compatible with Trackmate, that extracts Receptor Ligand Non-Equilibrium Kinetic (RLNEK) parameters from video-microscopy data. This software should be of particular interest to the community of researchers and scientists interrogating the target-specific binding and release of immune cells.

Keywords: 2D kinetics; Leukocyte; Shear flow; Surface-tethered reactants; Transmigration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ligands*

Substances

Ligands