Analysis of CCN Functions in Liver Regeneration After Partial Hepatectomy

Methods Mol Biol. 2023:2582:209-221. doi: 10.1007/978-1-0716-2744-0_14.

Abstract

The remarkable regenerative capability of the liver has long been appreciated. Upon significant loss of liver tissue, the remnant liver can grow rapidly to restore the original liver mass through a combination of hepatocyte proliferation and hypertrophy to maintain homeostasis. Experimentally, 2/3 partial hepatectomy in mice has been used extensively as a model to dissect the molecular mechanism of liver regeneration and the genetic networks involved. Herein, we describe the protocols for partial hepatectomy and analyses of pertinent CCN protein functions.

Keywords: Liver; Partial hepatectomy; Proliferation; Regeneration; Senescence; Surgery.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Proliferation
  • Hepatectomy* / methods
  • Hepatocytes / metabolism
  • Hyperplasia
  • Liver / surgery
  • Liver Regeneration*
  • Mice