Evaluation of hiPSC-Derived Muscle Progenitor Cell Transplantation in a Mouse Duchenne Muscular Dystrophy Model

Minas Nalbandian; Mingming Zhao; Hidetoshi Sakurai

doi:10.1007/978-1-0716-2772-3_28

Evaluation of hiPSC-Derived Muscle Progenitor Cell Transplantation in a Mouse Duchenne Muscular Dystrophy Model

Methods Mol Biol. 2023:2587:527-536. doi: 10.1007/978-1-0716-2772-3_28.

Authors

Minas Nalbandian¹, Mingming Zhao¹, Hidetoshi Sakurai²

Affiliations

¹ Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
² Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan. [email protected].

PMID: 36401048
DOI: 10.1007/978-1-0716-2772-3_28

Abstract

For cell therapy toward Duchenne muscle dystrophy (DMD), muscle progenitor cells derived from human-induced pluripotent stem cell (hiPSC-MuPCs) are recognized as a good candidate, and currently, cell transplantation of hiPSC-MuPCs is being tested with several DMD animal models. In this article, we describe an efficient method to dissociate, purify by cell sorting, transplant, and evaluate the transplantation efficacy of hiPSC-MuPCs.

Keywords: Cell therapy; DMD; Human iPS cells; Muscle stem cell; Skeletal muscle regeneration.

MeSH terms

Animals
Disease Models, Animal
Humans
Induced Pluripotent Stem Cells*
Mice
Muscles
Muscular Dystrophy, Duchenne* / therapy
Myoblasts
Stem Cell Transplantation