Generation and characterization of induced pluripotent stem (iPS) cell line (SDUCTi001-A) using fibroblasts derived from male-healthy donor

Morad Kamand; Sonja Hohmann; Sheena Louise Forsberg; Mads Thomassen; Mirolyuba Ilieva; Åsa Fex Svenningsen; Morten Meyer; Tanja Maria Michel

doi:10.1016/j.scr.2022.102961

Generation and characterization of induced pluripotent stem (iPS) cell line (SDUCTi001-A) using fibroblasts derived from male-healthy donor

Stem Cell Res. 2022 Dec:65:102961. doi: 10.1016/j.scr.2022.102961. Epub 2022 Nov 7.

Authors

Morad Kamand¹, Sonja Hohmann², Sheena Louise Forsberg², Mads Thomassen³, Mirolyuba Ilieva², Åsa Fex Svenningsen⁴, Morten Meyer⁵, Tanja Maria Michel⁶

Affiliations

¹ Department of Clinical Research, Department of Psychiatry, University of Southern Denmark, Odense, Denmark; Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark; Department of Epilepsy Genetics and Personalized Medicine, Danish Epilepsy Centre, Filadelfia, Denmark. Electronic address: [email protected].
² Department of Clinical Research, Department of Psychiatry, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark.
³ Department of Clinical Research, Department of Human Genetics, Odense University Hospital, Denmark.
⁴ Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark; Brain Research - Inter-Disciplinary Guided Excellence (BRIDGE), Odense, Denmark.
⁵ Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark; Brain Research - Inter-Disciplinary Guided Excellence (BRIDGE), Odense, Denmark; Department of Neurology, Odense University Hospital, Denmark.
⁶ Department of Clinical Research, Department of Psychiatry, University of Southern Denmark, Odense, Denmark; Brain Research - Inter-Disciplinary Guided Excellence (BRIDGE), Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark.

PMID: 36402078
DOI: 10.1016/j.scr.2022.102961

Abstract

Induced pluripotent stem (iPS) cell lines have wide valuable applications in experimental research, including developmental, pathological, and drug screening studies. Using integration-free episomal plasmids, we have generated a new iPS cell line from a 26-year-old healthy male donor. Characterization of the established cell line confirmed the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Line*
Humans
Male