Detection and Quantification of Protein Aggregates in Plants

Methods Mol Biol. 2023:2581:221-228. doi: 10.1007/978-1-0716-2784-6_15.

Abstract

Protein quality control is an important aspect of stress recovery. It maintains protein homeostasis through a machinery of regulatory proteins such as chaperones and proteases. When the system recognizes accumulation of misfolded or aggregated proteins, the cell recruits a set of regulatory proteins to initiate protein quality control. To understand the dynamics of stress-mediated aggregate protein formation and recovery in plants, robust methods aimed at detecting and measuring such protein aggregates are needed. This will help us to deepen our understanding of protein quality control mechanisms in plants.

Keywords: Aggregate proteins; Confocal microscopy; Plant protein quality control; Protein homeostasis; Volocity image analysis software.

MeSH terms

  • Heat-Shock Proteins / metabolism
  • Molecular Chaperones / metabolism
  • Plants* / metabolism
  • Protein Aggregates*
  • Protein Biosynthesis

Substances

  • Protein Aggregates
  • Molecular Chaperones
  • Heat-Shock Proteins