Case report: a genomics-guided reclassification of a blood culture isolate misassigned by MALDI-TOF as Yersinia pestis

Eby M Sim; Bryant Koh; Jimmy Ng; Trang Nguyen; Qinning Wang; Andrew N Ginn; Mitchell Brown; David Pham; Vitali Sintchenko

doi:10.1099/acmi.0.000422

Case report: a genomics-guided reclassification of a blood culture isolate misassigned by MALDI-TOF as Yersinia pestis

Access Microbiol. 2022 Oct 3;4(10):acmi000422. doi: 10.1099/acmi.0.000422. eCollection 2022.

Authors

Eby M Sim¹, Bryant Koh¹, Jimmy Ng¹, Trang Nguyen¹, Qinning Wang¹, Andrew N Ginn^{1

2}, Mitchell Brown¹, David Pham¹, Vitali Sintchenko^{1

2

3}

Affiliations

¹ Centre for Infectious Diseases and Microbiology- Laboratory Services, Institute of Clinical Pathology and Medical Research, NSW Health Pathology, Sydney, New South Wales, Australia.
² Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, New South Wales, Australia.
³ Centre for Infectious Diseases and Microbiology- Public Health, Westmead Hospital, Sydney, New South Wales, Australia.

Abstract

In this report, we describe a case where Gram-negative rods were isolated from a blood culture which subsequently presented a discordant Yersinia species result by MALDI-TOF. Rapid sequencing provided high-resolution identification of the isolate as Yersinia pseudotuberculosis , which was subsequently confirmed by biochemical tests.

Keywords: Yersinia; genome sequencing; laboratory biosafety; laboratory identification.

Publication types

Case Reports