Quantitative 1H NMR with global spectral deconvolution approach for the determination of gamma-aminobutyric acid in Chinese yam (Dioscorea polystachya Turczaninow)

Anal Sci. 2023 Feb;39(2):221-227. doi: 10.1007/s44211-022-00221-4. Epub 2022 Nov 24.

Abstract

We developed a quantitative proton nuclear magnetic resonance (qNMR) with global spectral deconvolution (GSD) method to determine the gamma-aminobutyric acid content in Chinese yam with the proton signal at δH 2.30. Trimethylsilyl-2,2,3,3-tetradeuteropropionic acid sodium salt was set as the internal standard. The method was validated and showed admissible stability, repeatability, and precision. Compared to the traditional high-performance liquid chromatography method, this method did not involve tedious pre-treatment and expensive standard. Compared to ordinary qNMR, GSD algorithm could effectively remove the effect of noise, baseline distortions and signal overlapping. Overall, qNMR with GSD method is a rapid, simple and reliable method to quantitatively determine functional metabolites even overlapped with other compounds in herbs or foods.

Keywords: Chinese yam; Functional food; Gamma-aminobutyric acid; Global spectral deconvolution; Quantitative 1H NMR.

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Dioscorea*
  • Magnetic Resonance Spectroscopy / methods
  • Proton Magnetic Resonance Spectroscopy
  • Protons

Substances

  • Protons