Comparing the effect of demineralized versus hybrid dentin matrices on inducing bone regeneration in New Zealand white rabbits' Mandibular defect

Objectives: The aim of this study was to compare the effect of using demineralized dentin matrix (DDM) versus hybrid dentin matrices; Demineralized and undemineralized dentin particles (DDM +UDDM) on inducing bone regeneration in mandibular defects.

Design: The study was conducted on fifty adult New Zealand rabbits, twenty for preparation of experimental materials and thirty for surgical procedures. They were randomly assigned into 3 equal groups as follow one control group: no treatment and two experimental groups including demineralized group: treated with DDM only; and hybrid group: treated with a hybrid of (50% DDM+50% UDD). A rounded critical size defect (10 mm in diameter- 5 mm in depth) was created in the body of mandible. After 3- and 6-weeks post-surgery, the bone regeneration was evaluated by light microscope, scanning electron microscope and histomorphometry.

Results: Histological, histomorphometrical observation and SEM revealed that both dentin matrices had largely resorbed and induced new bone formation at both experimental groups compared to the control group, with statistically higher percentage of new bone formation in the hybrid group.

Conclusion: We concluded that although both dentin matrices induced new bone formation; however, hybrid dentin matrix yielded better results compared to DDM group.