Generation and characterization of iPSCs from a patient with obsessive-compulsive disorder, his mother with schizophrenia and his healthy father

Xu Yuan Yin; Zhen Hua Zhu; Ru Feng Chen; Jian Li; Guang Ya Zhang; Wen Long Hou; Nan Nan Zhuang; Yuan Cai; Ning Bin Dai; Fang Liu; Jing Wang; Qiu Fang Jia; Li Hui

doi:10.1016/j.scr.2022.102993

Generation and characterization of iPSCs from a patient with obsessive-compulsive disorder, his mother with schizophrenia and his healthy father

Stem Cell Res. 2023 Feb:66:102993. doi: 10.1016/j.scr.2022.102993. Epub 2022 Dec 7.

Authors

Xu Yuan Yin¹, Zhen Hua Zhu¹, Ru Feng Chen¹, Jian Li², Guang Ya Zhang¹, Wen Long Hou¹, Nan Nan Zhuang¹, Yuan Cai¹, Ning Bin Dai³, Fang Liu³, Jing Wang¹, Qiu Fang Jia⁴, Li Hui⁵

Affiliations

¹ Research Center of Biological Psychiatry, Suzhou Psychiatric Hospital, Suzhou Medical College, Soochow University, Suzhou 215137, Jiangsu, China.
² Changshu No. 2 People's Hospital The Affiliated Changshu Hospital of Xuzhou Medical University, Changshu 215500, China.
³ Suzhou Center of Disease Control and Prevention, Suzhou 215004, RP China.
⁴ Research Center of Biological Psychiatry, Suzhou Psychiatric Hospital, Suzhou Medical College, Soochow University, Suzhou 215137, Jiangsu, China. Electronic address: [email protected].
⁵ Research Center of Biological Psychiatry, Suzhou Psychiatric Hospital, Suzhou Medical College, Soochow University, Suzhou 215137, Jiangsu, China. Electronic address: [email protected].

PMID: 36502622
DOI: 10.1016/j.scr.2022.102993

Abstract

Schizophrenia (SCZ) and obsessive-compulsive disorder (OCD) are complex polygenic disorders with brain morphology abnormalities. The etiologies and relationship of both disorders remain elusive, and should be further investigated. Thus, induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) from an OCD patient, his mother with SCZ and his healthy father with reprograming method. All iPSCs were characterized to have normal karyotype and expression of pluripotency makers. These iPSCs will be a valuable model to elucidate the pathophysiological mechanisms and association of both diseases.

Keywords: Induced pluripotent stem cells (iPSCs); Obsessive-compulsive disorder; Psychiatric disorders; Schizophrenia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fathers
Female
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear
Male
Mothers
Obsessive-Compulsive Disorder* / genetics
Obsessive-Compulsive Disorder* / metabolism
Schizophrenia* / genetics
Schizophrenia* / metabolism