Bond-selective intensity diffraction tomography

Jian Zhao; Alex Matlock; Hongbo Zhu; Ziqi Song; Jiabei Zhu; Biao Wang; Fukai Chen; Yuewei Zhan; Zhicong Chen; Yihong Xu; Xingchen Lin; Lei Tian; Ji-Xin Cheng

doi:10.1038/s41467-022-35329-8

Bond-selective intensity diffraction tomography

Nat Commun. 2022 Dec 15;13(1):7767. doi: 10.1038/s41467-022-35329-8.

Authors

Jian Zhao^#^{1

2}, Alex Matlock^#^{1

3}, Hongbo Zhu⁴, Ziqi Song⁵, Jiabei Zhu¹, Biao Wang⁶, Fukai Chen⁷, Yuewei Zhan⁸, Zhicong Chen¹, Yihong Xu⁹, Xingchen Lin⁶, Lei Tian^{10

11}, Ji-Xin Cheng^{12

13

14}

Affiliations

¹ Department of Electrical and Computer Engineering, Boston University, Boston, MA, 02215, USA.
² The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.
³ Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.
⁴ State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China. [email protected].
⁵ Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing, 100190, China.
⁶ State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China.
⁷ Department of Biology, Boston University, Boston, MA, 02215, USA.
⁸ Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA.
⁹ Department of Physics, Boston University, Boston, MA, 02215, USA.
¹⁰ Department of Electrical and Computer Engineering, Boston University, Boston, MA, 02215, USA. [email protected].
¹¹ Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA. [email protected].
¹² Department of Electrical and Computer Engineering, Boston University, Boston, MA, 02215, USA. [email protected].
¹³ Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA. [email protected].
¹⁴ Department of Physics, Boston University, Boston, MA, 02215, USA. [email protected].

^# Contributed equally.

Abstract

Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed a computational mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on a low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra and bond-selective 3D refractive index maps from intensity-only measurements. High-fidelity infrared fingerprint spectra extraction is validated. Volumetric chemical imaging of biological cells is demonstrated at a speed of ~20 s per volume, with a lateral and axial resolution of ~350 nm and ~1.1 µm, respectively. BS-IDT's application potential is investigated by chemically quantifying lipids stored in cancer cells and volumetric chemical imaging on Caenorhabditis elegans with a large field of view (~100 µm x 100 µm).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Microscopy* / methods
Tomography*