An 82-year-old woman was referred to our hospital because of severe mitral valve regurgitation( MR)with symptoms of heart failure. Preoperative transesophageal echocardiography( TEE) showed P2 prolapse due to chordal rupture, severe calcification of P2, and mild tricuspid valve regurgitation. The patient underwent mitral valve replacement using the MITRIS RESILIA mitral valve and tricuspid annuloplasty. Intraoperative TEE showed a mild regurgitation from the cuff on the A1P1 side at the mitral valve position. After the second aortic declamping, 4-0 prolene felted mattress suture was placed on the needle hole in the cuff. In repeat TEE, regurgitation improved to trace. Postoperative echocardiography confirmed disappearance of transprosthetic cuff leakage at the mitral valve, and the patient was discharged on postoperative day 36. We experienced a transprothetic cuff leakage, which is the first case on the MITRIS RESILIA mitral valve.