Visualization of pig sperm chromosomes by in-vitro penetration of zona-free hamster ova

J Reprod Fertil. 1987 Jul;80(2):619-22. doi: 10.1530/jrf.0.0800619.

Abstract

Zona-free hamster eggs were penetrated by pig spermatozoa capacitated using bovine follicular fluid and Percoll gradients. A mean penetration rate of 80.1% was obtained from 5 ejaculates from 2 boars. Penetrated eggs were cultured and analysable metaphase chromosome spreads were obtained from 16.8%. The analysis of 20 pig sperm complements indicated that 9 were 19, Y, 10 were 19, X and 1 appeared to have an XY sex chromosome constitution.

MeSH terms

  • Animals
  • Centrifugation, Density Gradient
  • Chromosomes / ultrastructure*
  • Cricetinae
  • Female
  • Male
  • Sperm-Ovum Interactions
  • Spermatozoa / ultrastructure*
  • Swine / genetics*
  • X Chromosome / ultrastructure
  • Y Chromosome / ultrastructure