The probability with which EMS-initiated mutagenic lesions generate mutations in CHO cells

Mutat Res. 1987 Sep;192(1):79-82. doi: 10.1016/0165-7992(87)90130-8.

Abstract

In this note we distinguish between multiple mutations affecting a given locus which are generated at separate error-prone lesions and multiple independent mutations generated at a single error-prone lesion. We describe a basis for determining the probability with which the latter class of mutations occurs based on the mutant fraction in the progeny and determine an average probability of 0.6 mutations/replication/mutagenic site for those EMS-modified sites which are mutagenic for G6PDH activity in CHO cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cricetinae
  • DNA / drug effects
  • DNA Damage*
  • DNA Repair
  • DNA Replication
  • Ethyl Methanesulfonate / toxicity*
  • Mutation / drug effects*
  • Probability

Substances

  • DNA
  • Ethyl Methanesulfonate