A protocol for collection and infusion of cerebrospinal fluid in mice

Achint Kaur; Steven Shuken; Andrew Chris Yang; Tal Iram

doi:10.1016/j.xpro.2022.102015

A protocol for collection and infusion of cerebrospinal fluid in mice

STAR Protoc. 2023 Mar 17;4(1):102015. doi: 10.1016/j.xpro.2022.102015. Epub 2023 Jan 12.

Authors

Achint Kaur¹, Steven Shuken¹, Andrew Chris Yang², Tal Iram³

Affiliations

¹ Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA; Wu Tsai Neurosciences Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
² Department of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA; Bakar Aging Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
³ Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA; Wu Tsai Neurosciences Institute, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: [email protected].

Abstract

Here, we provide a step-by-step protocol for the collection and intracerebroventricular infusion of cerebrospinal fluid (CSF) in mice. We describe steps to withdraw CSF quickly and abundantly while avoiding blood contamination. Using the Lynch coil technique, we gain functional insights into the collected CSF by slowly infusing minimal amounts of CSF directly to the lateral ventricles of aged mice. This protocol is versatile and can be used to infuse drugs, antibodies, or scarce biological compounds. For complete details on the use and execution of this protocol, please refer to Iram et al. (2022).¹.

Keywords: Model Organisms; Neuroscience.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies*
Infusions, Intraventricular
Mice

Substances

Antibodies

Grants and funding

RF1 AG064897/AG/NIA NIH HHS/United States