We report here, to the best of our knowledge, the first high-gain, single-frequency Tm3+-doped fiber amplifier operating at the 2.3-μm band with conventional ground-state pumping transition (3H6→3H4) at 793 nm. The gain fiber is an 8.5-m-long ZBLAN fiber with a Tm3+ doping concentration of 3 mol.%, and the seed is a single-frequency distributed feedback diode laser operated at 2331.9 nm. A gain up to 24.1 dB is generated for ∼14 W of launched pump power with the maximum output power of 246 mW. The competitive 3F4 →3H6 laser transition at ∼2 µm is also investigated, and the prospects for further power scaling are discussed.