Identification of 2-Methylthio-methylenethio-N6 -(cis-4-hydroxyisopentenyl)adenosine (msms2 io6 A37 ) as a Novel Modification at Adenosine 37 of tRNAs from Salmonella typhimurium

Marouane Libiad; Thierry Douki; Marc Fontecave; Mohamed Atta

doi:10.1002/cbic.202300019

Identification of 2-Methylthio-methylenethio-N⁶ -(cis-4-hydroxyisopentenyl)adenosine (msms² io⁶ A₃₇ ) as a Novel Modification at Adenosine 37 of tRNAs from Salmonella typhimurium

Chembiochem. 2023 Mar 14;24(6):e202300019. doi: 10.1002/cbic.202300019. Epub 2023 Feb 20.

Authors

Marouane Libiad¹, Thierry Douki², Marc Fontecave¹, Mohamed Atta^{3

1}

Affiliations

¹ Laboratoire de Chimie des Processus Biologiques, UMR 8229, Collège de France/CNRS/Sorbonne Université, 11, place Marcellin-Berthelot, Paris, France.
² Univ. Grenoble Alpes, CEA, CNRS, IRIG, SyMMES, 38000, Grenoble, France.
³ Univ. Grenoble Alpes, CEA, CNRS, IRIG, LCBM, UMR 5249, 38000, Grenoble, France.

PMID: 36640047
DOI: 10.1002/cbic.202300019

Abstract

Post-transcriptional modifications of tRNA nucleotide are important determinants in folding, structure and function. We have successfully identified and characterized a new modified base named 2-methylthio-methylenethio-N⁶ -(cis-4-hydroxyisopentenyl)adenosine, which is present at position 37 in some tRNAs. We also showed that this new modified adenosine is derived from the known 2-methylthio-methylenethio-N⁶ -(isopentenyl)adenosine nucleoside by a catalytic cycle of the tRNA-diiron monooxygenase, MiaE, present in Salmonella typhimurium.

Keywords: LC-MS; hydroxylation; tRNA modifications; tRNA-diiron monooxygenase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine*
Isopentenyladenosine / chemistry
Mixed Function Oxygenases / chemistry
RNA, Transfer / chemistry
Salmonella typhimurium* / genetics

Substances

Adenosine
RNA, Transfer
Isopentenyladenosine
Mixed Function Oxygenases