Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate

Methods Mol Biol. 2023:2625:231-240. doi: 10.1007/978-1-0716-2966-6_20.

Abstract

The bioactive sphingolipid sphingosine 1-phosphate (S1P) and its five cognate receptors (S1PR1-5) have been implicated to play important role in multiple aspects of human physiology and diseases. The S1P-S1PR1 signaling axis in endothelial cells is crucial for establishing flow competent blood vessels. The role of S1P in neovascular pathology is of great interest and is evolving as a promising target for treatment. Here we describe an easy and affordable in vivo model of corneal neovascularization using an alkali chemical burn to the cornea. This method gives a consistent and easy-to-quantitate procedure for neovascularization and angiogenesis studies.

Keywords: Angiogenesis; Cornea; Neovascularization; Sphingosine 1-phosphate.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cornea
  • Endothelial Cells*
  • Humans
  • Lysophospholipids
  • Receptors, Lysosphingolipid*
  • Sphingosine

Substances

  • sphingosine 1-phosphate
  • Receptors, Lysosphingolipid
  • Sphingosine
  • Lysophospholipids