Abstract
Background:
Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers.
Methodology:
To verify the expression of these proteins in tissue samples of AME, dentigerous cyst (DC) and dental follicle (DF), immunohistochemistry was performed and indirect immunofluorescence were performed on the human AME (AME-hTERT) cell line.
Results:
Revealed expression of SOX-2, NANOG and OCT4 in the tissue samples and AME-hTERT lineage. Greater immunostaining of the studied proteins was observed in AME compared to DC and DF (p < 0.001).
Conclusions:
The presence of biomarkers indicates a probable role of stem cells in the genesis and progression of AME.
Keywords:
Ameloblastoma; Immunohistochemistry; Octamer transcription factors; SOX transcription factors; Stem cells; Transcriptomic.
© 2023 Martins Balbinot et al.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Ameloblastoma* / genetics
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Ameloblastoma* / metabolism
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Biomarkers / metabolism
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism
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Cell Proliferation
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Humans
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Immunohistochemistry
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Nanog Homeobox Protein / genetics
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Neoplastic Stem Cells* / metabolism
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Octamer Transcription Factor-3 / genetics
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Octamer Transcription Factor-3 / metabolism
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SOXB1 Transcription Factors / genetics
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SOXB1 Transcription Factors / metabolism
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Stem Cells / metabolism
Substances
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Biomarkers, Tumor
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Nanog Homeobox Protein
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NANOG protein, human
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Biomarkers
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SOXB1 Transcription Factors
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Octamer Transcription Factor-3
Associated data
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figshare/10.6084/m9.figshare.19517200.v1
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figshare/10.6084/m9.figshare.19517263.v2
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figshare/10.6084/m9.figshare.21507090.v1
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figshare/10.6084/m9.figshare.21507096.v2
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figshare/10.6084/m9.figshare.21507102.v1
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figshare/10.6084/m9.figshare.21507117.v1
Grants and funding
The authors received funding from the National Council for Scientific and Technological Development, CNPq (Grants #305493/2018-3 and #435644/2018-1 awarded to Ricardo Alves Mesquita; Grant 307584/2018-6 awarded to Rommel T J Ramos; and Grant #429423/2018-7 awarded to João de Jesus Viana Pinheiro). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.