MIR-379-5p Expression in Endometrial Cancer and Its Correlation with ROR1 Expression

Asian Pac J Cancer Prev. 2023 Jan 1;24(1):239-248. doi: 10.31557/APJCP.2023.24.1.239.

Abstract

Objective: To asses miR-379-5p expression in endometrial cancer (EC) and its correlation with ROR1 expression and to investigate the relation between miR-379-5p and ROR1 expressions and the clinicopathological picture of EC.

Methods: Fifty female of EC were joined to this study. The gene expression of miR-379-5p (by quantitative real time-PCR) and ROR1 (by quantitative real time-PCR and immunohistochemistry) were studied in EC and normal nearby endometrial tissue.

Results: The gene expression of miR-379-5p was significantly downregulated while that of ROR1 was significantly upregulated in EC tissues compared to adjacent normal endometrial tissues. Furthermore, miR-379 and ROR1 expressions significantly associated with tumor stage (P< 0.045), grade (P< 0.001), myometrial invasion (P <0.001) and LN metastasis (P< 0.034). In addition, miR-3795p and ROR1 gene expression were negatively correlated (r = -0.746, P < 0.001).

Conclusions: In EC, miR-379-5p can be used as a diagnostic marker, and ROR1 could be a potential target of miR-379-5p.

Keywords: PCR; Prognosis; endometrial carcinoma; immunohistochemistry.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Endometrial Neoplasms* / pathology
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Receptor Tyrosine Kinase-like Orphan Receptors / genetics

Substances

  • MicroRNAs
  • ROR1 protein, human
  • Receptor Tyrosine Kinase-like Orphan Receptors
  • MIRN379 microRNA, human