FRET-based fluorescent probe with favorable water solubility for simultaneous detection of SO₂ derivatives and viscosity

The intracellular viscosity is an important parameter of the microenvironment and SO₂ is a vital gas signal molecule. At present, some dual-response fluorescence probes for simultaneous measurements of viscosity and SO₂ derivatives (HSO₃^-/SO₃^2-) possessed poor water solubility. In this work, we developed a water-soluble fluorescence probe CIJ (0.0864 g/100 mL of water at 20 °C) for simultaneous measurements of viscosity and SO₂ derivatives. CIJ exhibited a sensitive fluorescence enhancement to environmental viscosity from 0.97 to 28.04 cP based on a twisted intramolecular charge transfer mechanism and was applied to effective measurement of viscosity in vitro and in vivo. CIJ could also respond to SO₂ derivatives with a low detection limit (44 nM) and a fast response time (5 min) based on the nucleophilic addition reaction. Furthermore, CIJ was applied to monitor SO₂ derivatives in ratiometric response manner in living cells.