Regulation of probe density on upconversion nanoparticles enabling high-performance lateral flow assays

Birui Jin; Zhiguo Du; Jingcheng Ji; Yuemeng Bai; Deding Tang; Lihua Qiao; Jiatao Lou; Jie Hu; Zedong Li

doi:10.1016/j.talanta.2023.124327

Regulation of probe density on upconversion nanoparticles enabling high-performance lateral flow assays

Talanta. 2023 May 1:256:124327. doi: 10.1016/j.talanta.2023.124327. Epub 2023 Feb 4.

Authors

Birui Jin¹, Zhiguo Du², Jingcheng Ji³, Yuemeng Bai³, Deding Tang⁴, Lihua Qiao⁵, Jiatao Lou⁶, Jie Hu⁷, Zedong Li⁸

Affiliations

¹ School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an, 710021, China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, 710049, China.
² School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an, 710021, China.
³ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an, 710049, China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, 710049, China.
⁴ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an, 710049, China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, 710049, China; Maanshan Teachers College, Ma Anshan, 243041, China.
⁵ Department of Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, China.
⁶ Department of Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, China; Department of Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China.
⁷ Suzhou DiYinAn Biotech Co., Ltd., Suzhou Innovation Center for Life Science and Technology, Suzhou, 215129, China. Electronic address: [email protected].
⁸ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an, 710049, China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, 710049, China. Electronic address: [email protected].

PMID: 36758506
DOI: 10.1016/j.talanta.2023.124327

Abstract

Upconversion nanoparticles (UCNPs)-based fluorescence probes have shown great potential in point-of-care testing (POCT) applications, due to UCNPs' features of high photostability and background-free fluorescence. Ceaseless improvements of UCNPs-probes have been carried out to increase detection sensitivity and to broaden detection range of UCNPs-based POCT. In this paper, we optimized UCNPs-probes by regulating probe density. The optimization was verified by a traditional lateral flow assay (LFA) platform for C-reactive protein (CRP) detection. Further, the optimized UCNPs-LFA integrating with a home-made benchtop fluorescence analyzer holds the capability to achieve high-performance POCT. Finally, nearly a 20 times sensitivity enhancement with a limit of detection of 0.046 ng/mL and a broad detection range of 0.2-300 ng/mL for CRP detection was obtained. Moreover, the optimized UCNPs-LFA was applied to detecting CRP in clinical serum samples and the detection results were consistent with the clinical test, validating its clinical practicability. The proposed optimization method is also expected to optimize other nanoparticles-based bio-probes for wider POCT application.

Keywords: Bio-probe optimization; CRP detection; Lateral flow assay; Paper microfluidics; Sensitivity enhancement.

MeSH terms

C-Reactive Protein
Fluorescent Dyes
Limit of Detection
Nanoparticles*

Substances

Fluorescent Dyes
C-Reactive Protein