Objective: To analyze the expression and correlation of microRNA-195 (miR-195), miR-125 and calreticulin in diffuse large B-cell lymphoma (DLBCL).
Methods: From April 2020 to April 2021, 80 DLBCL patients with complete data archived by the Pathology Department of Handan First Hospital and The Second Hospital of Hebei Medical University were selected as the study group, and 70 patients with reactive lymph node hyperplasia were selected as the control group. The expressions of miR-195 and miR-125 were detected by real-time fluorescence quantitative PCR, and the expression of calreticulin was detected by Western blot. Pearson correlation was used to analyze the correlation between miR-195, miR-125, calreticulin and DLBCL, and ROC curve was used to analyze the predictive value of miR-195, miR-125 and calreticulin for DLBCL.
Results: Compared with the control group, the expression of miR-195 decreased but miR-125 and calreticulin increased in the study group (P<0.001). The expression levels of miR-195, miR-125 and calreticulin were not related to sex, age, primary site and B symptoms of patients with DLBCL, but related to immunophenotype, Ann Arbor stage, lactate dehydrogenase, IPI score, nodule involvement and Ki-67 index. The expression of miR-195 decreased and the expression of miR-125 and calreticulin increased in DLBCL paitents with non-germinal center source, Ann Arbor stage III-IV, lactate dehydrogenase > 245 U/L, IPI score 3-5, nodule involvement≥2 and Ki-67 index≥75% (P<0.05). Pearson correlation analysis showed that miR-195 and miR-125 were negatively correlated (r=-0.536, P=0.001), miR-195 and calreticulin were negatively correlated (r=-0.545, P=0.001), while miR-125 and calreticulin were positively correlated (r=0.523, P=0.001). ROC curve showed that compared with the single diagnosis of miR-195, miR-125 and calreticulin, the combination of the three items had higher predictive value for DLBCL (P<0.001).
Conclusion: The expression of miR-195 decreases and the expression of miR-125 and calreticulin increase in patients with DLBCL. Along with the increase of disease stage and IPI score, the decrease of miR-195 and the increase of miR-125 and calreticulin aggravate gradually. The three items may participate in the occurrence and progress of DLBCL.
题目: miR-195、miR-125和钙网蛋白在弥漫大B细胞淋巴瘤中的 表达及相关性研究.
目的: 分析微小RNA-195（miR-195）、miR-125和钙网蛋白在弥漫大B细胞淋巴瘤（DLBCL）中的表达及相关性.
方法: 选取2020年4月-2021年4月河北省邯郸市第一医院与河北医科大学第二医院病理科归档的资料齐全完整的80例DLBCL患者作为研究对象纳入研究组，另外选取70例淋巴结反应性增生患者纳入对照组。采用实时荧光定量PCR法检测miR-195、miR-125表达量，Western blot检测钙网蛋白表达情况，采用Pearson相关性分析法分析miR-195、miR-125和钙网蛋白与DLBCL之间的相关性，ROC曲线分析miR-195、miR-125和钙网蛋白对DLBCL的预测价值.
结果: 与对照组相比，研究组中miR-195表达量降低，miR-125、钙网蛋白表达量升高（P<0.001）。miR-195、miR-125和钙网蛋白的表达水平与患者性别、年龄、原发部位、B症状无关，与免疫表型、Ann Arbor临床分期、乳酸脱氢酶、IPI评分、结节累及、Ki-67指数有关，非生发中心来源、Ann Arbor分期为Ⅲ-Ⅳ期、乳酸脱氢酶>245 U/L、IPI评分3-5分、结节累及≥2处、Ki-67指数≥75%的DLBCL患者miR-195表达水平降低，miR-125、钙网蛋白表达水平升高（P<0.05）。Pearson相关性分析显示，miR-195与miR-125呈负相关（r=-0.536），miR-125与钙网蛋白呈负相关（r=-0.545）；miR-125与钙网蛋白呈正相关（r=0.523）。ROC曲线显示，与miR-195、miR-125和钙网蛋白单项诊断相比，三项联合对DLBCL的预测价值较高（P<0.001）.
结论: miR-195在DLBCL中表达降低，miR-125、钙网蛋白表达升高，且随着疾病的分期与IPI评分的升高，miR-195降低加剧，miR-125、钙网蛋白升高加剧，三者可能共同参与DLBCL的发生进展.
Keywords: calreticulin; diffuse large B-cell lymphoma; microRNA-125; microRNA-195.