Polyploid SNP Genotyping Using the MassARRAY System

Aline da Costa Lima Moraes; Danilo Augusto Sforça; Melina Cristina Mancini; Bianca Baccili Zanotto Vigna; Anete Pereira de Souza

doi:10.1007/978-1-0716-3024-2_7

Polyploid SNP Genotyping Using the MassARRAY System

Methods Mol Biol. 2023:2638:93-113. doi: 10.1007/978-1-0716-3024-2_7.

Authors

Aline da Costa Lima Moraes¹, Danilo Augusto Sforça², Melina Cristina Mancini², Bianca Baccili Zanotto Vigna³, Anete Pereira de Souza^{4

5}

Affiliations

¹ Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, Brazil.
² Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, Brazil.
³ Embrapa Pecuária Sudeste, Brazilian Agricultural Research Corporation, São Carlos, Brazil.
⁴ Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, Brazil. [email protected].
⁵ Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, Brazil. [email protected].

PMID: 36781637
DOI: 10.1007/978-1-0716-3024-2_7

Abstract

Molecular marker discovery and genotyping are major challenges in polyploid breeding programs incorporating molecular biology tools. In this context, this work describes a method for single nucleotide polymorphism (SNP) genotyping in polyploid crops using matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry, the MassARRAY System.

Keywords: Allele dosage; Genotyping; MassARRAY; Polyploid; Single nucleotide polymorphism (SNP).

MeSH terms

Genotype
Plant Breeding*
Polymorphism, Single Nucleotide*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods