Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-Based Proteomic Identification

Amirmohammad Nasiri Kenari; Linda Bojmar; Søren Heissel; Henrik Molina; David Lyden; Ayuko Hoshino

doi:10.1007/978-1-0716-2978-9_19

Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-Based Proteomic Identification

Methods Mol Biol. 2023:2628:291-300. doi: 10.1007/978-1-0716-2978-9_19.

Authors

Amirmohammad Nasiri Kenari¹, Linda Bojmar², Søren Heissel³, Henrik Molina³, David Lyden², Ayuko Hoshino⁴

Affiliations

¹ School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan.
² Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA.
³ Proteomics Resource Center, The Rockefeller University, New York, NY, USA.
⁴ School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan. [email protected].

PMID: 36781793
DOI: 10.1007/978-1-0716-2978-9_19

Abstract

Plasma extracellular vesicles and particles (EVPs) are enriched in biomolecules that reflect individuals' physiological and pathological states. Several studies have demonstrated the potential of human plasma EVPs as a novel liquid biopsy. Here we describe a protocol for human plasma EVPs isolation and proteomic characterization. We isolated human plasma EVPs by the classical ultracentrifugation method and performed mass spectrometry-based proteomic profiling. Using this protocol, researchers can reveal the plasma EVPs proteome and explore the clinical application of plasma EVPs proteins for developing disease biomarkers.

Keywords: Extracellular vesicles and particles; Mass spectrometry; Plasma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood Proteins / metabolism
Extracellular Vesicles* / metabolism
Humans
Mass Spectrometry
Proteome / metabolism
Proteomics* / methods
Ultracentrifugation

Substances

Blood Proteins
Proteome