CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa

Luisa Galindo-Sotomonte; Cintia Jozefkowicz; Cristina Gómez; Margarita Stritzler; Romina Frare; Emilia Bottero; Hiromi Tajima; Eduardo Blumwald; Nicolas Ayub; Gabriela Soto

doi:10.1007/s00299-023-02997-9

CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa

Plant Cell Rep. 2023 May;42(5):953-956. doi: 10.1007/s00299-023-02997-9. Epub 2023 Feb 25.

Authors

Luisa Galindo-Sotomonte^#^{1

2}, Cintia Jozefkowicz^#^{1

2}, Cristina Gómez^{1

2}, Margarita Stritzler^{1

2}, Romina Frare^{1

2}, Emilia Bottero^{1

2}, Hiromi Tajima³, Eduardo Blumwald³, Nicolas Ayub^{1

2}, Gabriela Soto^{4

5}

Affiliations

¹ Instituto de Agrobiotecnología y Biología Molecular (IABIMO, CONICET-INTA), Buenos Aires, Argentina.
² Instituto de Genética (IGEAF, INTA), Buenos Aires, Argentina.
³ Department of Plant Sciences, University of California, Davis, CA, 95616, USA.
⁴ Instituto de Agrobiotecnología y Biología Molecular (IABIMO, CONICET-INTA), Buenos Aires, Argentina. [email protected].
⁵ Instituto de Genética (IGEAF, INTA), Buenos Aires, Argentina. [email protected].

^# Contributed equally.

PMID: 36840757
DOI: 10.1007/s00299-023-02997-9

Abstract

T-DNA and CRISPR/Cas9-mediated knockout of polyester synthase-like genes delays flowering time in Arabidopsis thaliana and Medicago sativa (alfalfa). Thus, we here present the first report of edited alfalfa with delayed flowering.

Keywords: Alfalfa; Arabidopsis; CRISPR; Flowering; Vegetative growth.

MeSH terms

Arabidopsis* / genetics
CRISPR-Cas Systems / genetics
Flowers / genetics
Medicago sativa* / genetics

Substances

poly(3-hydroxyalkanoic acid) synthase

Abstract

MeSH terms

Substances

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