The genome sequence of the malaria mosquito, Anopheles funestus, Giles, 1900

Diego Ayala; Ousman Akone-Ella; Pierre Kengne; Harriet Johnson; Haynes Heaton; Joanna Collins; Ksenia Krasheninnikova; Sarah Pelan; Damon-Lee Pointon; Ying Sims; James Torrance; Alan Tracey; Marcela Uliano-Silva; Katharina von Wyschetzki; Jonathan Wood; DNA Pipelines collective; Shane McCarthy; Daniel Neafsey; Alex Makunin; Mara Lawniczak

doi:10.12688/wellcomeopenres.18445.2

The genome sequence of the malaria mosquito, Anopheles funestus, Giles, 1900

Wellcome Open Res. 2023 Mar 27:7:287. doi: 10.12688/wellcomeopenres.18445.2. eCollection 2022.

Authors

Diego Ayala^{1

2}, Ousman Akone-Ella², Pierre Kengne^{1

2}, Harriet Johnson³, Haynes Heaton⁴, Joanna Collins⁵, Ksenia Krasheninnikova⁵, Sarah Pelan⁵, Damon-Lee Pointon⁵, Ying Sims⁵, James Torrance⁵, Alan Tracey⁵, Marcela Uliano-Silva⁵, Katharina von Wyschetzki⁵, Jonathan Wood⁵; DNA Pipelines collective; Shane McCarthy^{5

6}, Daniel Neafsey^{7

8}, Alex Makunin⁵, Mara Lawniczak⁵

Affiliations

¹ MIVEGEC, IRD, Montpellier, 34394, France.
² ESV-GAB, Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville, BP 769, Gabon.
³ Scientific Operations, Wellcome Sanger Institute, Hinxton, CB10 1SA, UK.
⁴ CSSE, Auburn University, Auburn, AL, 36849, USA.
⁵ Tree of Life, Wellcome Sanger Institute, Hinxton, CB10 1SA, UK.
⁶ Department of Genetics, University of Cambridge, Cambridge, CB2 3EH, UK.
⁷ Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, 02115, USA.
⁸ Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, 02142, USA.

Abstract

We present a genome assembly from an individual female Anopheles funestus (the malaria mosquito; Arthropoda; Insecta; Diptera; Culicidae). The genome sequence is 251 megabases in span. The majority of the assembly is scaffolded into three chromosomal pseudomolecules with the X sex chromosome assembled. The complete mitochondrial genome was also assembled and is 15.4 kilobases in length.

Keywords: African malaria mosquito; Anopheles funestus; chromosomal inversions; genome sequence.

Grants and funding

This work was supported by a Bill & Melinda Gates Foundation Award (INV-009760) to MKNL. The Wellcome Sanger Institute is funded by Wellcome (206194, https://doi.org/10.35802/206194), which supports MKNL. Field collection was supported by an ANR grant (ANR-18-CE35-0002-01 –WILDING). SAM is supported from a Wellcome Grant (207492, https://doi.org/10.35802/207492).