Magnetic three-phase single-drop microextraction for highly sensitive detection of aflatoxin B1 in agricultural product samples based on peroxidase-like spatial network structure

Jingyu Kuang; Jiahe Ju; Yongli Lu; Yitong Chen; Chang Liu; Dezhao Kong; Wei Shen; Hai-Wei Shi; Li Li; Jin Ye; Sheng Tang

doi:10.1016/j.foodchem.2023.135856

Magnetic three-phase single-drop microextraction for highly sensitive detection of aflatoxin B₁ in agricultural product samples based on peroxidase-like spatial network structure

Food Chem. 2023 Aug 1:416:135856. doi: 10.1016/j.foodchem.2023.135856. Epub 2023 Mar 4.

Authors

Jingyu Kuang¹, Jiahe Ju¹, Yongli Lu², Yitong Chen¹, Chang Liu², Dezhao Kong³, Wei Shen⁴, Hai-Wei Shi⁵, Li Li⁶, Jin Ye⁶, Sheng Tang⁷

Affiliations

¹ School of Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China.
² School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China.
³ School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China. Electronic address: [email protected].
⁴ School of Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China. Electronic address: [email protected].
⁵ Jiangsu Institute for Food and Drug Control, Nanjing 210019, Jiangsu Province, PR China; School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China.
⁶ Academy of National Food and Strategic Reserves Administration, Beijing 100037, PR China.
⁷ School of Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China. Electronic address: [email protected].

PMID: 36898338
DOI: 10.1016/j.foodchem.2023.135856

Abstract

In this work, a highly sensitive method for aflatoxin B₁ (AFB₁) detection was developed based on a peroxidase-like spatial network structure. The specific antibody and antigen of AFB₁ were coated on a histidine-modified Fe₃O₄ nanozyme to form the capture/detection probes. Based on the competition/affinity effect, the spatial network structure was constructed by the probes, which could be rapidly (8 s) separated by a magnetic three-phase single-drop microextraction process. In this single-drop microreactor, the network structure was applied to catalyze a colorimetric 3,3',5,5'-tetramethylbenzidine oxidation reaction for AFB₁ detection. The signal was amplified significantly due to the strong peroxidase-like ability of the spatial network structure and the enrichment effect of the microextraction. Thus, a low detection limit (0.034 pg/mL) was achieved. The matrix effect of real sample can be eliminated by the extraction approach, and the practicability of this method was proved by agricultural product samples analysis.

Keywords: Aflatoxin B(1); Magnetic three-phase single-drop microextraction; Nanozyme; Spatial network structure.

MeSH terms

Aflatoxin B1 / analysis
Biosensing Techniques* / methods
Coloring Agents / analysis
Food Contamination / analysis
Limit of Detection
Magnetic Phenomena
Peroxidase*
Peroxidases

Substances

Peroxidase
Aflatoxin B1
Peroxidases
Coloring Agents