Protocol for in vivo measurement of basal and insulin-stimulated glucose uptake in mouse tissues

Meng Zhao; Lianna W Wat; Katrin J Svensson

doi:10.1016/j.xpro.2023.102179

Protocol for in vivo measurement of basal and insulin-stimulated glucose uptake in mouse tissues

STAR Protoc. 2023 Mar 17;4(2):102179. doi: 10.1016/j.xpro.2023.102179. Online ahead of print.

Authors

Meng Zhao¹, Lianna W Wat¹, Katrin J Svensson²

Affiliations

¹ Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA; Stanford Diabetes Research Center, Stanford University School of Medicine, Stanford, CA 94305, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
² Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA; Stanford Diabetes Research Center, Stanford University School of Medicine, Stanford, CA 94305, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: [email protected].

Abstract

Here, we present an in vivo protocol for measuring basal and insulin-stimulated glucose uptake in tissues from mice. We describe steps for administering 2-deoxy-D-[1,2-³H]glucose in the presence or absence of insulin via intraperitoneal injections. We then detail tissue collection, tissue processing to measure ³H counts on a scintillation counter, and data interpretation. This protocol can be applied to other glucoregulatory hormones, genetic mouse models, and other species. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).¹.

Keywords: Metabolism; Molecular/Chemical Probes.

Grants and funding

R01 DK125260/DK/NIDDK NIH HHS/United States