Preparation of the individual diastereomers of adenylyl-(2'-5')-P-thioadenylyl-(2'-5')-adenosine and their 5'-phosphorylated derivatives

E de Vroom; A Fidder; C P Saris; G A van der Marel; J H van Boom

doi:10.1093/nar/15.23.9933

Preparation of the individual diastereomers of adenylyl-(2'-5')-P-thioadenylyl-(2'-5')-adenosine and their 5'-phosphorylated derivatives

Nucleic Acids Res. 1987 Dec 10;15(23):9933-43. doi: 10.1093/nar/15.23.9933.

Authors

E de Vroom¹, A Fidder, C P Saris, G A van der Marel, J H van Boom

Affiliation

¹ Gorlaeus Laboratory, Leiden, The Netherlands.

Abstract

The individual diastereomers of trimer A2'p5'A2'p(s)5'A, containing one phosphorothioate linkage, were prepared via a modified hydroxybenzotriazole phosphotriester approach. The 5'-phosphorylated derivatives of the latter compounds were obtained after phosphorylation with a 6-trifluoromethyl-1-benzotriazolyl activated phosphoromorpholidate.

MeSH terms

Adenine Nucleotides / chemical synthesis*
Cross-Linking Reagents
Nucleic Acid Conformation
Oligoribonucleotides / analogs & derivatives
Oligoribonucleotides / chemical synthesis*
Phosphorylation
Stereoisomerism

Substances

Adenine Nucleotides
Cross-Linking Reagents
Oligoribonucleotides
2',5'-oligoadenylate