Estimation, Evaluation and Characterization of Carbapenem Resistance Burden from a Tertiary Care Hospital, Pakistan

Aamir Jamal Gondal; Nakhshab Choudhry; Hina Bukhari; Zainab Rizvi; Shah Jahan; Nighat Yasmin

doi:10.3390/antibiotics12030525

Estimation, Evaluation and Characterization of Carbapenem Resistance Burden from a Tertiary Care Hospital, Pakistan

Antibiotics (Basel). 2023 Mar 6;12(3):525. doi: 10.3390/antibiotics12030525.

Authors

Aamir Jamal Gondal¹, Nakhshab Choudhry², Hina Bukhari³, Zainab Rizvi⁴, Shah Jahan⁵, Nighat Yasmin¹

Affiliations

¹ Department of Biomedical Sciences, King Edward Medical University, Lahore 54000, Pakistan.
² Department of Biochemistry, King Edward Medical University, Lahore 54000, Pakistan.
³ Department of Pathology, King Edward Medical University, Lahore 54000, Pakistan.
⁴ Department of Oral Pathology, de'Montmorency College of Dentistry, Lahore 54000, Pakistan.
⁵ Department of Immunology, University of Health Sciences, Lahore 54600, Pakistan.

Abstract

Carbapenem resistance has become major concern in healthcare settings globally; therefore, its monitoring is crucial for intervention efforts to halt resistance spread. During May 2019-April 2022, 2170 clinical strains were characterized for antimicrobial susceptibility, resistance genes, replicon and sequence types. Overall, 42.1% isolates were carbapenem-resistant, and significantly associated with Klebsiella pneumoniae (K. pneumoniae) (p = 0.008) and Proteus species (p = 0.043). Carbapenemases were detected in 82.2% of isolates, with bla_NDM-1 (41.1%) associated with the ICU (p < 0.001), cardiology (p = 0.042), pediatric medicine (p = 0.013) and wound samples (p = 0.041); bla_OXA-48 (32.6%) was associated with the ICU (p < 0.001), cardiology (p = 0.008), pediatric medicine (p < 0.001), general surgery (p = 0.001), general medicine (p = 0.005) and nephrology (p = 0.020); bla_KPC-2 (5.5%) was associated with general surgery (p = 0.029); bla_NDM-1/bla_OXA-48 (11.4%) was associated with general surgery (p < 0.001), and wound (p = 0.002), urine (p = 0.003) and blood (p = 0.012) samples; bla_OXA-48/bla_VIM (3.1%) was associated with nephrology (p < 0.001) and urine samples (p < 0.001). Other detected carbapenemases were bla_VIM (3.0%), bla_IMP (2.7%), bla_OXA-48/bla_IMP (0.1%) and bla_VIM/bla_IMP (0.3%). Sequence type (ST)147 (39.7%) represented the most common sequence type identified among K. pneumoniae, along with ST11 (23.0%), ST14 (15.4%), ST258 (10.9%) and ST340 (9.6%) while ST405 comprised 34.5% of Escherichia coli (E. coli) isolates followed by ST131 (21.2%), ST101 (19.7%), ST10 (16.0%) and ST69 (7.4%). Plasmid replicon types IncFII, IncA/C, IncN, IncL/M, IncFIIA and IncFIIK were observed. This is first report describing the carbapenem-resistance burden and emergence of bla_KPC-2-ST147, bla_NDM-1-ST340 and bla_NDM-1-ST14 in K. pneumoniae isolates and bla_NDM-1-ST69 and bla_NDM-1/bla_OXA-48-ST69 in E. coli isolates coharboring extended-spectrum beta-lactamases (ESBLs) from Pakistan.

Keywords: Enterobacterales; Pakistan; carbapenem resistance; carbapenemases; sequence types.

Grants and funding

41/RC/KEMU/King Edward Medical University