The recombinant DNA-derived 20,000-dalton variant of N-terminal methionyl human growth hormone (20K-Met-hGH) had a hyperglycemic effect in fasted dogs when injected 11 hours prior to an oral glucose tolerance test (OGTT). These results reported here suggest that 20K-Met-hGH can induce glucose intolerance in dogs similar to that produced by recombinant DNA-derived 22,000 dalton N-terminal methionyl human growth hormone (22K-Met-hGH) and the normal pituitary-source human growth hormone (22K-hGH).