Derivation of Bovine Primed Embryonic Stem Cells from Somatic Cell Nuclear Transfer Embryos

Methods Mol Biol. 2023:2647:305-315. doi: 10.1007/978-1-0716-3064-8_17.

Abstract

Derivation of bovine embryonic stem cells from somatic cell nuclear transfer embryos enables the derivation of genetically matched pluripotent stem cell lines to valuable and well-characterized animals. In this chapter, we describe a step-by-step procedure for deriving bovine embryonic stem cells from whole blastocysts produced by somatic cell nuclear transfer. This simple method requires minimal manipulation of blastocyst-stage embryos, relies on commercially available reagents, supports trypsin passaging, and allows the generation of stable primed pluripotent stem cell lines in 3-4 weeks.

Keywords: Blastocyst; Derivation; Embryonic stem cells; Inner cell mass; Pluripotency; Somatic cell nuclear transfer.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / metabolism
  • Cattle
  • Embryo Culture Techniques / methods
  • Embryonic Stem Cells* / metabolism
  • Nuclear Transfer Techniques
  • Pluripotent Stem Cells* / metabolism