Derivation of bovine embryonic stem cells from somatic cell nuclear transfer embryos enables the derivation of genetically matched pluripotent stem cell lines to valuable and well-characterized animals. In this chapter, we describe a step-by-step procedure for deriving bovine embryonic stem cells from whole blastocysts produced by somatic cell nuclear transfer. This simple method requires minimal manipulation of blastocyst-stage embryos, relies on commercially available reagents, supports trypsin passaging, and allows the generation of stable primed pluripotent stem cell lines in 3-4 weeks.
Keywords: Blastocyst; Derivation; Embryonic stem cells; Inner cell mass; Pluripotency; Somatic cell nuclear transfer.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.