Interaction of 2-dodecylcyclobutanone (2-DCB) and internal standard in irradiated chicken jerkies and pig ears in solid-phase microextraction (SPME)-coupled gas chromatography-mass spectrometry (GC-MS) analysis

The radiolysis of palmitic acid in chicken jerky (CJ) and pig ears (PE) can form 2-dodecylcyclobutanone (2-DCB). A solid-phase micro extraction-coupled GC-MS technique can be used for quantitative analysis of 2-DCB with the adequate use of an internal standard (IS). The objectives of this study are to: 1) investigate the IS and 2-DCB interactions as a function of IS concentration and irradiation dose; 2) elucidate the effects of bound 2-DCB; 3) use electron paramagnetic resonance spectroscopy to complement 2-DCB measurements. The measurement of 2-DCB formed by irradiation was significantly (P < 0.05) affected by the palmitic acid content and IS concentration. The amount of 2-DCB measured in irradiated (10 kGy) CJ and PE increased 70% and 300%, respectively, when the IS concentration increased from 8 to 800 ppb. Our findings serve as a guide for the adequate use of IS for quantitative analysis of 2-DCB formed in irradiated meat matrixes.