Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations

Methods Mol Biol. 2023:2652:361-379. doi: 10.1007/978-1-0716-3147-8_21.

Abstract

Time-resolved serial crystallography is an emerging method to elucidate the structure-function relationship of biomolecular systems at up to atomic resolution. However, to make this demanding method a success, a number of experimental requirements have to be met. In this chapter, we summarize general guidelines and protocols towards performing time-resolved crystallography experiments, with a particular emphasis on sample requirements and preparation but also a brief excursion into reaction initiation.

Keywords: SSX; Serial crystallography; Structural biology; Structural enzymology; Time-resolved crystallography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallography / methods
  • Crystallography, X-Ray
  • Specimen Handling*
  • Time Factors