Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model

Nanxi Lai; Zhisheng Li; Zhong Chen; Yi Wang

doi:10.1016/j.xpro.2023.102255

Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model

STAR Protoc. 2023 Apr 25;4(2):102255. doi: 10.1016/j.xpro.2023.102255. Online ahead of print.

Authors

Nanxi Lai¹, Zhisheng Li¹, Zhong Chen², Yi Wang³

Affiliations

¹ Institute of Pharmacology & Toxicology, NHC and CAMS Key Laboratory of Medical Neurobiology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
² Institute of Pharmacology & Toxicology, NHC and CAMS Key Laboratory of Medical Neurobiology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China; Key Laboratory of Neuropharmacology and Translational Medicine of Zhejiang Province, School of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China; Epilepsy Center, Department of Neurology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 313000, China. Electronic address: [email protected].
³ Institute of Pharmacology & Toxicology, NHC and CAMS Key Laboratory of Medical Neurobiology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China; Key Laboratory of Neuropharmacology and Translational Medicine of Zhejiang Province, School of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China; Epilepsy Center, Department of Neurology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 313000, China. Electronic address: [email protected].

Abstract

Epileptic networks are characterized by two states, seizures or more prolonged interictal periods. Here, we present the procedure for labeling seizure-activated and interictal-activated neuronal ensembles in mouse hippocampal kindling model using an enhanced-synaptic-activity-responsive element. We describe the seizure model establishment, tamoxifen induction, electrical stimulation, and calcium signal recording of labeled ensembles. This protocol has demonstrated dissociated calcium activities in the two ensembles during focal seizure dynamics and can be applied to other animal models of epilepsy. For complete details on the use and execution of this protocol, please refer to Lai et al. (2022).¹.

Keywords: Behavior; Neuroscience.