Liquid exfoliation of bulk g-C₃N₅ to nanosheets for improved photocatalytic antibacterial activity

Liquid exfoliation of bulk g-C₃N₅ was applied to synthesize g-C₃N₅ nanosheets. In order to characterize the samples, X-ray powder diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectra (FT-IR), X-ray photoelectron spectra (XPS), UV-Vis absorption spectra (UV-Vis), and photoluminescence spectra (PL) were examined. g-C₃N₅ nanosheets exhibited enhanced performance in the inactivation of Escherichia coli (E. coli) with visible light irradiation relative to bulk g-C₃N₅ and promoted complete inactivation of E. coli within 120 min. h⁺ and •O₂^- were the principal reactive species in the antibacterial process. In the early stages, SOD and CAT played a defensive role in resisting oxidative damage of active species. With the prolonged light exposure time, the antioxidant protection system was overwhelmed leading to the destruction of the cell membrane. The leakage of cell contents such as K⁺, protein, and DNA caused bacterial apoptosis ultimately. The enhanced photocatalytic antibacterial performance of g-C₃N₅ nanosheets is ascribed to the stronger redox property by the upward shift of CB and downward shift of VB compared with bulk g-C₃N₅. On the other hand, larger specific surface area and better separation efficiency of photoinduced carriers are helpful to the improved photocatalytic performance. This study systematically revealed the inactivation process toward E. coli and expanded the application range of g-C₃N₅-based materials with abundant solar energy.